How to set-up molecular diagnostics lab or biotechnology lab

How to set-up molecular diagnostics lab or biotechnology lab

If you are setting up or expanding an existing lab to include RT-PCR assays, at a minimum, your lab will need these small laboratory equipment items to prepare and perform tests for various infectious diseases like COVID-19 PCR tests. A basic biotechnology lab setup typically needs the following equipment:

  1. RT-PCR (Polymerase Chain Reaction) machine or Thermocycler and Gel Electrophoresis
  2. Deep Freezer (-80 degrees)
  3. Freezer (-20 degrees)
  4. BioSafety Cabinet
  5. Laminar Airflow
  6. Refrigerated Centrifuge
  7. Vortex Mixer
  8. Binocular Microscope
  9. Incubator
  10. Autoclave/ sterilizer
  11. Micro-centrifuge
  12. Refrigerator

However, to establish a successful medical biotechnology lab, adequate space with proper layout, appropriate equipment and qualified personnel are a must. This is because PCR testing is vulnerable to cross contamination, especially from external DNA sources like hair and body fluids from technicians’ itself.

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